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Within the vitro follicle incubation that have radiolabeled steroid precursors

Within the vitro follicle incubation that have radiolabeled steroid precursors
Seafood and you can testing

In spawning 12 months (late booleaf wrasse was in fact caught because of the link and line inside the seaside waters close to the Fisheries Look Lab, Kyushu College or university and gone to live in the brand new research. Fish were stored in five-hundred-litre fiberglass tanks which have blocked seawater, under absolute big date-size and you will liquids temperature, and you can provided krill and you may real time hermit crab once a day. After verifying each day spawning, cuatro–6 female fish (lbs – g, complete length 11step three–159 mm) was sampled during the , , , and you can hr. Fish were anesthetized which have 2-phenoxyethanol (300 ppm), and bloodstream samples have been amassed on caudal ship having fun with syringes suitable which have 25-grams for 20 min. The fresh new broke up solution try stored during the ?30°C up to assayed to possess steroid top. After blood testing, fish were slain because of the decapitation, while the ovaries was dissected out. Continue reading